Bovine Viral Diarrhea Virus Type 1&2 (BVDV-1&2) MAb Ascites E2 gp53 IgG1 Isotype
Binds to the E2 (gp53) protein of Bovine Viral Diarrhea Virus Type 1 (BVDV-1) and to Bovine Viral Diarrhea Virus Type 2 (BVDV-2). IgG1 isotype. Can be used as a reagent to detect both BVDV-1 and BVDV-2 using indirect immunofluoresence or indirect immunoperoxidase reactions. Does not work with formalin-fixed tissues. This monoclonal antibody is produced as mouse ascites fluid, clarified by centrifugation, and filtered through a 0.2 micrometer filter. The concentration is 1.0 mg/ml in phosphate-buffered saline, preserved with sodium azide. This antibody is stabilized by the addition of 4 mg/ml BSA. Note: BVDV-1 genotypes usually produce only mild diarrhea in immunocompetent cattle, while BVDV-2 genotypes can produce thrombocytopenia, hemorrhages, and acute fatal disease. The viruses of either genotype may exist as one of two biotypes, cytopathic or noncytopathic.
Quantity
Species
Bovine
Shelf Life
One year from date of sale
Storage
Store at 2-7°C. DO NOT FREEZE!
Size
0.1 mg
Origin
Mouse ascites
Infectious Agent
Bovine Viral Diarrhea
Known Applications
Immunofluorescence, Indirect immunoperoxidase
Restrictions
N/A
Antibodies
Deregt, D., et al. Mapping of a type 1-specific and a type-common epitope on the E2 (gp53) protein of bovine viral diarrhea virus with neutralization escape mutants. Virus Research 53(1):81-90 (Jan. 1998).
Deregt, D., and S. Prins. A monoclonal antibody-based immunoperoxidase layer (micro-isolation) assay for detection of type 1 and type 2 bovine viral diarrhea viruses. Can. J. Vet. Res. 62(2):152-155 (Apr. 1998).
Deregt, D., et al. Monoclonal antibodies to the E2 protein of a new genotype (type 2) of bovine viral diarrhea virus define three antigenic domains involved in neutralization. Virus Res. 57(2):171-181 (Oct. 1998).

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