Bovine Leukemia Antibody Test Kit, ELISA
Detection of antibodies to Bovine Leukemia Virus (BLV) glycoprotein 51 (gp51) in bovine sera by ELISA. For Veterinary Use Only - Use Only As Directed
Quantity
Species
Bovine
Sample Type
Serum
Sensitivity
98.0%
Specificity
100.0%
Shelf Life
One year from date of QC release
Storage
Store at 2-8°C. DO NOT FREEZE!
Restrictions
N/A
Configuration
2 stripwell plates
Format
cELISA
Tests
182
Assay Time
60 minutes
Related Products
This Bovine Leukemia Virus Antibody Test Kit is an enzyme-linked, immunosorbent assay (ELISA). Sample serum antibodies bind to BLV gp51 molecules attached to the plastic wells of the microtiter plate. Binding of these serum antibodies is detected by reaction with horseradish peroxidase (HRP)-labeled affinity-purified goat antibodies to bovine immunoglobulins. Attached HRP-labeled antibodies are detected by addition of enzyme substrate and quantitated by subsequent blue color product development. Strong color development indicates the presence of antibody to BLV gp51 in the sample serum. Very weak or no color development indicates the absence of antibody to BLV gp51 in the sample serum.
About Bovine Leukosis
Enzootic Bovine Leukosis (EBL) is an infectious, non-contagious viral disease of cattle. It is caused by BLV, an oncogenic delta retrovirus, which results in proliferation of B lymphocytes. Infection with BLV may lead to persistent lymphocytosis and in some adult cattle to the development of tumors with associated signs. The spread of disease from the introduction into a herd may reach enzootic proportions. Transmission of BLV occurs between animal primarliy by transfer of B lymphocytes in blood. Trauma, use of common breeding needles, and surgical procedures are the main means of transmission. It is rarely vertically transmitted. Most BLV infections are inapparent with approximately 5% of animals developing clinical signs. AGID and ELISA tests are used to identify carrier cattle. Control programs for EBL include testing and removal of positive animals. Several European countries which have instituted eradication programs also require that imported cattle be free of BLV.
Technical Assistance
Our technical support team is available to assist as needed. You can reach technical support for products at support@vmrd.com.