Bluetongue Virus Antibody Test Kit, AGID
Detection of antibody to Bluetongue Virus (BTV) and Epizootic Hemorrhagic Disease (EHD) virus in ruminant serum by AGID. For Veterinary Use Only - Use Only As Directed
Quantity
Species
Bovine, Camelid, Cervine, Caprine, Ovine
Sample Type
Serum
Sensitivity
100.0%
Specificity
99.0%
Shelf Life
Twenty-four months from date of QC release
Storage
Store at 2-8°C. DO NOT FREEZE!
Restrictions
N/A
Configuration
N/A
Format
AGID
Tests
100
Assay Time
30 minutes
Related Products
This Bluetongue Virus antibody Test Kit is an agar-gel immunodiffusion (AGID) assay that takes place in agarose using the 7-well standard procedure described by Pearson and Jochim (American Association of Veterinary Laboratory Diagnosticians, 22nd Annual Proceedings, 463-471, 1979). Soluble bluetongue virus antigen is placed in the center well and positive reference serum is placed in three alternating peripheral wells. Sample sera are placed in the 3 remaining wells. After incubation reference lines form between the antigen well and the reference serum wells. Sample sera, if positive, will form a line that fuses with reference lines or that deviates the positive reference lines inward near the sample well without formation of a visible line. Negative sera will neither form a line nor cause deviation of the reference lines.
About Bluetongue Virus
Bluetongue is an infectious, non-contagious, arthropodborne, viral disease of wild and domestic ruminants. In cattle it is usually a subclinical infection, while in sheep it is often characterized by acute catarrhal inflammation of mucous membranes and hyperemia of coronary bands. Degenerative changes are present in skeletal and coronary musculature,leading to weakness, prolonged convalescence and significant economic losses.
Bluetongue virus (BTV) belongs to the genus Orbivirus, family Reoviridae. Laboratory diagnosis of bluetongue is primarily established by isolation of the virus. Virus is isolated in Vero or BHK 21 cells, and its presence is confirmed by immunofluorescence. Serological methods used in diagnosis of this disease are AGID, ELISA, cELISA and immunofluorescence. Positive results confirm exposure to BTV but not necessarily carrier status.
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