Bluetongue Virus Antibody Test Kit, cELISA v2
Detection of antibody to Bluetongue Virus in ruminant serum by cELISA. Does not detect antibody to Epizootic Hemorrhagic Disease (EHD). For Veterinary Use Only - Use Only As Directed
Quantity
Species
Bovine, Camelid, Cervine, Caprine, Ovine
Sample Type
Serum
Sensitivity
100.0%
Specificity
99.0%
Shelf Life
Eighteen months from date of QC release
Storage
Store at 2-8°C. DO NOT FREEZE!
Restrictions
N/A
Configuration
2 stripwell plates
Format
cELISA
Tests
184
Assay Time
40 minutes
Related Products
VMRD’s new BTV cELISA v2 test kit brings you the same rapid and convenient features of the original version (40 minutes total incubation time) along with new features including distilled water wash.
This Bluetongue Virus Antibody Test Kit is a competitive, enzyme-linked, immunosorbent assay (cELISA). Sample serum bluetongue antibody inhibits binding of horseradish peroxidase (HRP)-labeled, bluetongue-specific monoclonal antibody to bluetongue viral antigen coated on the plastic wells. Binding of the HRP-labeled monoclonal antibody conjugate is detected by the addition of enzyme substrate and quantified by subsequent color product development. Strong color development indicates little or no blockage of HRP-labeled monoclonal antibody binding and therefore the absence of bluetongue antibody in sample sera. Weak color development due to inhibition of the monoclonal antibody binding to the antigen on the solid phase indicates the presence of bluetongue antibodies in sample sera.
About Bluetongue Virus
Bluetongue is an infectious, non-contagious, arthropodborne, viral disease of wild and domestic ruminants. In cattle it is usually a subclinical infection, while in sheep it is often characterized by acute catarrhal inflammation of mucous membranes and hyperemia of coronary bands. Degenerative changes are present in skeletal and coronary musculature,leading to weakness, prolonged convalescence and significant economic losses.
Bluetongue virus (BTV) belongs to the genus Orbivirus, family Reoviridae. Laboratory diagnosis of bluetongue is primarily established by isolation of the virus. Virus is isolated in Vero or BHK 21 cells, and its presence is confirmed by immunofluorescence. Serological methods used in diagnosis of this disease are AGID, ELISA, cELISA and immunofluorescence. Positive results confirm exposure to BTV but not necessarily carrier status.
Technical Assistance
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