Neospora caninum Antibody Test Kit, cELISA
Detection of antibodies to Neospora caninum in bovine serum by cELISA. For Veterinary Use Only - Use Only As Directed
Quantity
Species
Bovine
Sample Type
Serum
Sensitivity
96.0%
Specificity
99.0%
Shelf Life
Twenty-four months from date of QC release
Storage
Store at 2-8°C. DO NOT FREEZE!
Restrictions
N/A
Configuration
2 stripwell plates
Format
cELISA
Tests
184
Assay Time
100 minutes
Related Products
This Neospora caninum Antibody Test Kit is a competitive, enzyme-linked, immunosorbent assay (cELISA). Sample serum N. caninum antibody inhibits binding of horseradish peroxidase (HRP)-labeled N. caninum-specific monoclonal antibody to N. caninum tachyzoite antigen coated on the plastic wells. Binding of the HRP-labeled monoclonal antibody conjugate is detected by the addition of enzyme substrate and quantified by subsequent color product development. Strong color development indicates little or no blockage of HRP-labeled monoclonal antibody binding and therefore the absence of N. caninum antibody in sample sera. Weak color development due to inhibition of the monoclonal antibody binding to the antigen on the solid phase indicates the presence of N. caninum antibodies in sample sera.
About Neosporosis
Neosporosis has been identified across the world in various species, including dogs, cattle, sheep, goats, and horses. It is caused by Neospora caninum, a protozoan parasite closely related to Toxoplasma gondii. Although canids have been identified as the definitive host for N. caninum, it is not known if there are other definitive hosts. No clinical signs are noted in cows that abort due to N. caninum either prior to the abortion or post-abortion. Aborted fetuses are usually autolyzed with no
gross lesions and placentas are not retained. Abortions have been diagnosed in both heifers and cows from 3 months gestation to term. A majority (78%) of N. caninum abortions occur between 4 and 6 months gestation. This pattern of mid-gestation abortion is distinct from other diagnosed causes of infectious abortion in dairy cattle which tend to occur later in gestation. In dogs, N. caninum infection causes neuromuscular paralysis. Identification of carrier animals is based upon detection of specific antibody with serological tests while diagnosis of abortions is based upon microscopic examination of the fetus and immunohistochemistry.
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