A complete collection of molecular assays, ELISA kits and extraction tools now supported by VMRD’s 45‑year history of reliable veterinary diagnostics and customer service. Transition Changes Products are now available to order through us. Orders can be placed via phone, email, or by submitting an online quote request. During the transition period, orders placed with us will continue to ship from Tetracore's facility until manufacturing has transitioned to us. Orders placed through us will be invoiced by us and payable to us. Previous orders placed with Tetracore and invoiced by Tetracore remain payable to Tetracore. Customer service and technical support are available through us, with Tetracore assisting behind the scenes. Interested in learning more? Learn More Tetracore Diagnostic Products Now Offered Through VMRD A complete collection of molecular assays, ELISA kits, and extraction tools now supported by VMRD's 45-year history of reliable veterinary & customer service. View Products NEW ITEM EZ-Universal Flu A 2.1 Real-Time RT-PCR Add to Quote Request NEW ITEM ASF 3.0 Dry Real-Time RT-PCR Assay with Inhibition and Endogenous Control Add to Quote Request NEW ITEM FMDV 2.1 Dry Real-Time RT-PCR Assay with Internal Controls Add to Quote Request NEW ITEM African Swine Fever Virus (ASFV) Oral Fluids Antibody Test Kit Indirect ELISA Add to Quote Request NEW ITEM African Swine Fever Virus (ASFV) Serum Antibody Test Kit Indirect ELISA Add to Quote Request NEW ITEM EZ-Series Complete Positive Control Add to Quote Request NEW ITEM EZ-B. hyo/hamp/pilo MPX 1.0 Real-Time RT-PCR Assay Add to Quote Request NEW ITEM EZ-Series Internal Control Add to Quote Request NEW ITEM RNA Internal Control System Add to Quote Request NEW ITEM DNA Internal Control System Add to Quote Request NEW ITEM MagBead Total Nucleic Acid Extraction Kit Add to Quote Request NEW ITEM EZ-M Hyo Real-Time RT-PCR Assay Add to Quote Request NEW ITEM EZ-SVA 1.1 Real-Time RT-PCR Assay Add to Quote Request NEW ITEM EZ-Universal Flu A 2.1 Real-Time RT-PCR Assay Add to Quote Request NEW ITEM EZ-PED and PED INDEL Differential Assay Add to Quote Request NEW ITEM EZ-PED/TGE/PDCoV MPX 1.2 Add to Quote Request NEW ITEM EZ-PED/TGE/PDCoV MPX 1.2 Add to Quote Request NEW ITEM EZ-PRRSV/Flu A MPX 2.0 Add to Quote Request NEW ITEM EZ-PRRSV™ MPX 5.0 (with ROX) Add to Quote Request NEW ITEM EZ-PRRSV™ MPX 5.0 (No ROX) Add to Quote Request NEW ITEM MAP Extraction System Add to Quote Request NEW ITEM CSF Dry Real-Time RT-PCR Assay with Internal Control Add to Quote Request NEW ITEM WNV 2.1 Real-Time RT-PCR Assay Add to Quote Request Tetracore and VMRD have partnered to transition a group of trusted animal health products into VMRD's catalog. This change is designed to be simple and fully supported for current users. Contact VMRD for support with Tetracore molecular animal health products, ordering, shipping, and technical questions. CONTACT US Download Customer Communication and Product List DOWNLOAD Transition Partnership

VMRD manufactures veterinary diagnostics including USDA-licensed test kits and reagents for a variety of animal disease, as well as offers Testing Services for raw materials and seeds for the presence of adventitious agents. Molecular Animal Health Products Now Offered Through VMRD A complete collection of molecular assays, ELISA kits, and extraction tools now supported by VMRD's 45-year history of reliable veterinary diagnostics and customer service. Learn More A NEW HORSE-SIDE TEST FOR INSULIN IS NOW AVAILABLE! VMRD Launches a whole blood test for insulin with numerical results in 15 minutes. Learn More Avian 9CFR Regulatory Testing Learn More ECOMMERCE IS HERE!! Our Point-of-Care Products are Now Available to Purchase Online! Learn More Introducing the ELISAProXL A NEW HIGH-CAPACITY Automated ELISA Processor is Now Available Learn More VMRD+ READER NOW AVAILABLE! Now with RECHARGEABLE BATTERY! Learn More NEW & AVAILABLE! CWD RT-QuIC Reagents! VMRD has partnered with CWD Evolution to manufacture RT-QuIC reagents for detection of prions causing CWD Learn More Nucleic Acid Extraction Kits Available We are proud to announce the addition of Nucleic Acid Extraction Kits to our Molecular Product Line... Learn More Technical Library Products Catalog Career Openings Biologics Testing Services Catalog Explore VMRD Use our technical library to enhance your scientific knowledge related to our products and services, discover opportunities to work with us, or download a copy of our most recent catalog. VMRD Products The VMRD portfolio is the most comprehensive in veterinary diagnostics and has a wide range of products that include USDA-licensed ELISA kits, FA reagents, antibodies, and Coombs testing reagents. View Products for Veterinarians View Products for Laboratories Biologics Testing VMRD's Testing Services division (formerly ABR) specializes in testing of raw materials and seeds for the presence of adventitious agents to satisfy various regulatory requirements and quality assurance needs for the global serum, veterinary and pharmaceutical industries. View Services

USDA-licensed test kits, FA reagents, antibodies, RT-QuIC reagents, and coombs reagents, as well as automated solutions such as the ELISApro automated ELISA processor. Products for Laboratories Your trusted partner for veterinary diagnostic kits, reagents, automation solutions, and more. Automation ELISAPro Automated ELISA Processor ELISAProXL Automated ELISA Processor iPRO Automated FA Processor Software & Programs Diagnostics Diagnostic Test Kits FA Reagents Antibodies Immunology Reagents RT-PCR EZ-PRRS™ MPX 5.0 (No ROX) EZ-PRRS™ MPX 5.0 (with ROX) Learn More RT-QuIC for Chronic Wasting Disease (CWD) CWD Amplification Reagent CWD 5X Reaction Buffer CWD 10X Sample Dilution Buffer RT-QuIC Synthetic Positive Control Learn More Nucleic Acid Extraction Magnetic Bead Nucleic Acid Extraction Kit Magnetic Separator Lysis Beads ExtractPro Lysis Solution Learn More Need a custom product? We can do that! Show Me Need serology reference testing? We can do that also! Show Me Diagnostics Diagnostic Test Kits FA Reagents Antibodies Immunology Reagents Automation ELISAPro Automated ELISA Processor iPRO Automated FA Processor Software & Programs Nucleic Acid Extraction Magnetic Bead Nucleic Acid Extraction Kit Magnetic Separator Lysis Beads Learn More RT-QuIC for Chronic Wasting Disease (CWD) CWD Amplification Reagent CWD 5X Reaction Buffer CWD 10X Sample Dilution Buffer Learn More

See the ELISAProXL High-Capacity Automated ELISA Processor in action!

See the iPRO Automated IFA Processor in action!

Tetracore, Inc., and VMRD, Inc., announced today that Tetracore's animal health products have been purchased by VMRD. A complete list of products is available below. Both organizations are working closely to ensure this transition is smooth and supported for customers. Product descriptions, catalog numbers, and performance characteristics will remain the same. “Our shared priority is the customer experience,” said JJ McCallie, Vice President of Products. “We are committed to delivering the products our customers rely on with the same high-quality they've come to expect.” During the transition period, orders placed through VMRD will continue to ship from Tetracore’s facility. This partnership approach ensures uninterrupted supply while VMRD prepares inventory and production within its Pullman, Washington operations. “Tetracore has always valued the trust our customers place in these products,” said Dr. Nelson, President & CEO. “Working directly with VMRD allows us to make this transition easy and predictable for everyone.” Customer service and technical support will be handled by VMRD, with Tetracore assisting behind the scenes. About VMRD VMRD has supported veterinarians, laboratories, and animal‑health professionals for 45 years with reliable diagnostic tools and responsive customer service. About Tetracore Tetracore develops and supplies high‑quality diagnostic reagents and assays for animal health and biodefense applications. Media Contacts: JJ McCallie, Vice President of Products james.mccallie@vmrd.com (509) 610-7520 Dr. Bill Nelson (240) 268-5400

Summary The VMRD Insulin test is a lateral flow assay that offers objective quantitation of equine insulin values in whole blood using the VMRD Reader, also used for VMRD SAA and Foal IgG. As with other VMRD patient-side tests, this assay is calibrated against an accepted reference test, which in this case is the radioimmunoassay (RIA) offered by the Cornell Animal Health Diagnostic Center (AHDC). Every test lot is individually calibrated for maximum quality control to ensure consistency and accuracy of results over time and between lots. The calibration system keeps results consistent over time using RFID cards that are programmed with specific details for each lot and attached to the side of the reader when running the test. Tests must be stored refrigerated (2-8°C) and then warmed to room temperature before use. For accurate results they must be run at a temperature between 60-85°F (15-30°C), otherwise results may be higher (high temperatures) or lower (low temperatures) than expected. Although temperatures outside that range are often encountered in the field, results will be most reliable if these guidelines can be followed, particularly when tracking values for an individual over time. Each test comes with all components to run the test. The specific volume of blood sample needed is collected using a sampling pipette and then diluted in buffer. Five drops of diluted sample are applied to the test cartridge, and the test is run for 15 minutes either using the self-time function on the reader or timed by the user. Results are then interpreted and displayed by the reader, with numerical values reported between 20-200 μIU/mL. The VMRD Insulin test gives veterinarians the right information to provide immediate results and recommendations to owners for effective care and management of horses with insulin dysregulation (ID), allowing them to tailor management strategies to the needs of the individual horse. Comparison to RIA reference standard Excellent correlation was observed between the VMRD Insulin test and the reference test for general field samples and samples taken during oral sugar tests for horses with ID. Field samples The insulin concentrations of 58 field samples from 27 equids (24 horses and 3 mules) were determined with reference testing at the Cornell AHDC, then compared with the results from the VMRD Insulin lateral flow test. This sample set included seven individuals with diagnosed ID, each with multiple oral sugar test timepoints. Three to five test replicates were performed for each sample. Correlation of lateral flow with RIA for these samples was excellent with an R² value of 0.966. (Figure 1) Figure 1 Oral sugar tests Low-dose oral sugar tests were performed on seven horses suspected of having ID but whose baseline insulin concentrations were below the clinical cutoff of 45 μIU/mL (1). (Figure 2) All seven horses were diagnosed with ID and subsequently considered at high risk of hyperinsulinemia-associated laminitis according to the Equine Endocrinology EMS Guidelines (1). Figure 2 Dilution curve Using samples collected during the course of an oral sugar test, equine blood with a moderately high insulin concentration (45 min sample) was diluted at various ratios with blood containing a low concentration of insulin (”Pre” sample) from the same horse. Each dilution was tested in triplicate by the same user, and plasma was harvested and sent to the Cornell ADHC to characterize the exact insulin concentrations. Across the tested range of 34 to 150 μIU/mL, the VMRD Insulin test had excellent correlation with the reference test, with an R² value of 0.995. (Figure 3) Figure 3 Test variability Intra- and inter-assay variation Two lots of tests were compared with one technician running 10 replicates from each lot on six samples ranging in concentration from 14 to 116 uIU/mL. For intra-assay variation, CVs ranged from 6 to 14% for Lot A (average 10%) and from 8 to 13% for Lot B (average 11%). Inter-assay variation showed CVs ranging between 9 and 16% across all samples when encompassing data from both lots, with an average CV of 13%. (Table 1) This demonstrates good consistency between lots, with variation comparable to that observed in results obtained through submission to the reference lab (see below). Table 1 Inter-runner variability Variation was further evaluated by two technicians using three blood samples of various concentrations (14, 38, and 78 μIU/mL) as determined by Cornell. Each technician ran five replicates, for a total of 10 replicates of each sample. Variation ranged from 7-11% with an average variation of 9.3%. Reference RIA test variation To evaluate the inherent variability of the reference test used for calibration and validation of the VMRD Insulin test, 15 plasma samples (8 individual and 7 pooled) of varying concentrations were submitted to the Cornell ADHC for testing by RIA. Samples were tested in three separate runs on different days, and %CV ranged from 5-21%. (Table 2) This demonstrates that the level of variability observed with the VMRD Insulin test is also consistent with what can be expected from submission of samples for laboratory testing. Since the insulin values of each sample were defined by this reference test, no further assessment could be made regarding the accuracy of each value. Table 2 Conclusion The VMRD Insulin test correlates very well with the gold standard RIA test run by the Cornell ADHC, providing accurate, quantitative results using the VMRD Reader. Results for both normal and ID horses were clinically accurate across a broad range of insulin concentrations and supported rapid diagnosis of ID in multiple horses used for this study. Assessment of test variability demonstrated consistent results between runners and between lots, comparing favorably to the RIA reference test. The VMRD Insulin test offers a new, high-quality option for horse-side measurement of equine insulin in whole blood, without the delay and hassle of sending out samples or having to spin down samples and run them in a lab space. Testing horse-side enables dynamic assessment of insulin values to immediately evaluate the effect of current feeding and management strategies and offer timely recommendations for the prevention of hyperinsulinemia-associated laminitis. References Equine Endocrinology Group. (2024). Recommendations of diagnosis and management of equine metabolic syndrome (EMS), including assessment of insulin status. https://equineendocrinologygroup.org/ Learn more about VMRD Insulin

Donkey rescue operations face unique challenges that require specialized knowledge and tools. At Oscar’s Place, a sanctuary caring for around 250 donkeys across two locations in California, the daily work involves more than just sheltering these animals. It includes managing herd health, preventing disease outbreaks, and providing urgent care when needed. We recently had the chance to speak with the supervising veterinarian at Oscar’s Place about how they approach donkey care, especially their use of Serum Amyloid A (SAA) testing to detect illness early in a population known for hiding symptoms. Can you tell us a bit about Oscar’s Place and the scope of donkey care you manage day to day? Oscar’s Place is a donkey rescue that houses ≈250 donkeys currently between our two locations in Hopland and Potter Valley, California. Our donkeys come to us via two different routes—rescued from slaughter via the auction pipeline and also from loving families who are no longer able to care for them. Once in our care, we address any health issues they have and then work to rehabilitate them either into our general population to live out their lives in our pastures or adopt them out to new families seeking donkey companions. My work as the supervising veterinarian involves a mix of population medicine—herd health, preventive care, and disease mitigation—as well as urgent/emergent care for illness and injury that arise. With cases coming from auctions and other communal housing environments, this involves a lot of infectious disease surveillance and biosecurity practices in addition to treatment of sick donkeys in order to protect the existing herd. What initially drew you to using SAA testing, particularly for a large and diverse donkey population? I leaned heavily on SAA in my previous jobs—both in private practice and as the equine ambulatory clinician at Oregon State. When you are out in the field and need to triage/make decisions on the farm, having the insight of SAA in conjunction with my physical exam and imaging diagnostics is hugely helpful. Similarly here in Hopland, we want prompt answers to diagnostic questions so that we can initiate prompt care. Donkeys are amazingly stoic and often do not reveal their illness until they are very sick—anything that can help us detect disease sooner rather than later is tantamount to maintaining a healthy herd. Were there any early questions or hesitations about using SAA in donkeys versus horses? Yes! I have struggled to find literature that reassures me values can be trusted and interpreted in donkeys comparably to horses. Not all equids are equal. Donkeys have different reference intervals and physiological responses to disease that can be different than horses. It still involves a little bit of a leap of faith to “rely” on SAA readings, but as always, we use it as a part of clinical assessment and hope that the data we are collecting over time can provide more certainty in the values as we go along. How are you currently using SAA testing for screening and monitoring across your donkey population? When donkeys arrive from long distances, we perform SAA testing (among other things) on them at intake as well as at scheduled intervals over time until they recover. This is part of how we are building a better understanding of SAA response in donkeys but also trying to detect disease when clinical signs aren’t manifesting outwardly (as donkeys “hide their feelings” well). Additionally, we run SAA as part of our minimum database collection on any donkey that presents with respiratory symptoms, fever, diarrhea, and/or the “ADR” condition. Frequently, we use the SAA to track response to treatment thereafter as well. How has SAA testing helped with intake, quarantine, or managing new groups of donkeys coming into the herd? With rescues, we track the SAA at regular intervals on the whole rescued herd (sometimes 10 - 45 donkeys). We collect this information in conjunction with vital signs and thoracic ultrasound imaging. It helps direct treatment decisions as well as flags donkeys who may need additional diagnostics/closer monitoring. Recently we were also able to track viral loads of common respiratory pathogens in donkeys in both nasal swabs and whole blood at the same time points as our SAA readings. I am hopeful this data can help reveal a better understanding of the diseases as well as the donkey’s inflammatory response. Have you noticed any patterns in how SAA behaves in donkeys during treatment and recovery? Overall, my impression is that SAA trends are comparable in donkeys to the horses of my past experience. This is very anecdotal, but SAA does seem a little slower to rise as high and decrease than it did in horses, but perhaps that is just the nature of the diseases we are seeing here versus in horses (largely pneumonia—both viral and bacterial). I can say that with viral pneumonias, the SAAs have been minimally elevated, and in the later phases of bacterial pneumonia (or even before they recover), SAA values have been <20. Generally, when the fevers are still lingering, the SAAs are still high. Can you share an example of how a normal or low SAA result has been helpful? A normal or low SAA after an upward trend is very reassuring in the course of antibiotic therapy. It is also reassuring when you anticipate the disease is viral rather than bacterial and it seems to peak and drop faster (in my experience) with viral vs. continuing to rise with bacterial. Because we have had some unfortunate experiences with circulating herpesviruses here, those trends are very helpful, as we can’t always have PCR testing confirmed as quickly as we’d like (especially on a weekend or holiday!). What would you say to other donkey rescues or veterinarians who are considering SAA testing but aren’t sure yet? As with any diagnostic, it must be taken into account with the whole clinical picture, but for a species that likes to fog that clinical picture with the utmost stoicism and masking of disease, SAA can be your best detective friend. Is there anything else you’d like to share about how diagnostics like SAA support better welfare outcomes for donkeys? The more we utilize it and other diagnostics to establish norms and reference parameters, the better we will understand donkeys! Donkeys deserve to be better understood. Want to connect with the team at Oscar's Place? You can find them at: Oscar's Place Adoption Center & Sanctuary 4425 Hwy 175 Hopland, CA 95449 Phone: 707-530-6711 Website: oscarsplace.org