This Magnetic Bead Nucleic Acid Extraction Kit is suitable for manual or automated extraction of nucleic acids from a wide range of veterinary sample types. Purified RNA/DNA from viruses, bacteria, and parasites are ready-for-use in downstream reactions. Starting sample material may include bodily fluids such as blood, serum, plasma, semen, milk, and saliva (oral fluids), as well as swabs, feces, and tissues. 

The principles behind Magnetic Bead Extraction are Lyse, Bind/Adsorb, Separate, Wash, Elute. In the first step, nucleic acids are released from the sample upon lysis with the lysis master mix (SLS or ELS) containing Proteinase K. Binding Buffer and paramagnetic beads are next added to the lysate to facilitate nucleic acid binding to the beads. The magnetic beads are then separated from the remaining sample material by applying a magnetic field to the sample tubes or plate. (In automated plate systems, the beads are collected with 96 magnetized rods/pins.) After magnetic separation, unbound contaminants and salts are washed away from the beads/nucleic acids with Wash Buffers 1 and 2, then 80% ethanol. Residual ethanol is removed by brief air drying of the separated beads. Purified nucleic acids are eluted from the magnetic beads under low salt conditions.