Prion Protein MAb Ascites IHFG IgG1 Isotype
F89/160.1.5
Recognizes a conserved epitope (IHFG) on the prion protein in tissues from sheep, cattle, mule deer, elk, white tailed deer and humans. IgG1 isotype. Suitable for detecting agents of TSEs in ruminant species. Techniques include immunoassasys of formalin-fixes tissues, Western immunoblot, immunohistochemistry (except in cervid lymphoid tissue - F99/97.6.1), and ELISA. F89-160.1.5 (Lot F89-008) was evaluated by immunohistochemistry (IHC) of brain and lymph node from a scrapie-infected sheep, and brain and lymph node from a sheep with no known exposure to scrapie. The antibody was diluted to 0.5 µg/ml and incubated for 30 minutes with pretreated tissues. Dectection was performed on a Ventana immunostainer using biotinylated goat anti-mouse Ig. Streptavidin-horseradish peroxidase and AEC as the chromogen/substrate. There was no staining of the tissue form the sheep with no exposure to scrapie. Staining of the brain and lymph node of the scrapie-infected sheep was intense. The quality of this lot of antibody is excellent. Tissues for IHC with this antibody are paraffin-embedded formalin-fixed tissues, often decontaminated by incubation of formalin-fixed tissue in formic acid for 60 minutes before re-equilibration in formalin and routine processing and embedding. Three to 5 micron sections are rehydrated, Pretreated first with acid (98% formic acid 5-20 minutes, then rinsed and neutralized with several changes of 0.1 M Tris buffer, pH 7.5) then with heat (autoclaved 121°C for 20 minutes in 0.1 M Tris buffer, Ph 7.5 or in modified citrate buffer, pH 6.1, such as DAKO Target Retrieval Buffer).
SHELF LIFE:
One year from date of sale
STORAGE:
Store at 2-7°C. Do not freeze!
SIZE:
0.1 mg
ORIGIN:
Mouse Ascites
INFECTIOUS AGENT:
transmissible spongiform encephalopathy
RESTRICTIONS:
Customer must have TSE Statement on file for the current year.
KNOWN APPLICATIONS:
Immunoassays, Western immunoblot, Immunohistochemistry, ELISA
Technical Assistance
Our technical support team is available to assist as needed. You can reach technical support for products at support@vmrd.com.
Sigurdson, C.J., et al. Oral transmission and early lymphoid tropism of chronic wasting disease PrPres in mule deer fawns (Odocoileus hemionus). J. Gen. Virol. 80(10):2757-2764 (Oct. 1999).
Ersdal, C., et al. Accumulation of pathogenic prion protein (PrPSc) in nervous and lymphoid tissues of sheep with subclinical scrapie. Vet. Pathol. 40(2):164-174 (Mar. 2003).
O'Rourke, K.I., et al. Monoclonal antibody F89/160.1.5 defines a conserved epitope on the ruminant prion protein. J. Clin. Microbiol. 36(6):1750-1755 (June 1998).
Onnasch, H., et al. Two Irish cases of scrapie resembling Nor98. Vet. Record155(20):636-637 (Nov. 2004).
O'Rourke, K.I., et al. Preclinical detection of PrPSc in nictitating membrane lymphoid tissue of sheep. Vet. Rec. 142(18):489-491 (May 1998).
Spraker, T.R., et al. Comparison of histological lesions and immunohistochemical staining of proteinase-resistant prion protein in a naturally occurring spongiform encephalopathy of free-ranging mule deer (Odocoileus hemionus) with those of chronic wasting disease of captive mule deer. Vet. Pathol. 39(1):110-119 (Jan. 2002).
Koo, H.C., et al. Immunohistochemical detection of prion protein (PrP-Sc) and epidemiological study of BSE in Korea. J. Vet. Sci. 2(1):25-31 (Apr. 2001).
Spraker, T.R., et al. Distribution of protease-resistant prion protein and spongiform encephalopathy in free-ranging mule deer (Odocoileus hemionus) with chronic wasting disease. Vet. Pathol. 39(5):546-556 (Sept. 2002).
O'Rourke, K.I., et al. Preclinical diagnosis of scrapie by immunohistochemistry of third eyelid lymphoid tissue. J. Clin. Microbiol. 38(9):3254-3259 (Sept. 2000).
Van Everbroeck, B., et al. Immunoreactivity of the monoclonal antibody F89/160.1.5 for the human prion protein. Eur. J. Histochem. 43(4):335-338 (1999).
Jeffrey, M., et al. Ovine infection with the agents of scrapie (CH1641 isolate) and bovine spongiform encephalopathy: Immunochemical similarities can be resolved by immunohistochemistry. J. Comp. Pathol. 134(1):17-29 (Jan. 2006)
