Canine Distemper Virus (CDV) MAb Ascites IgG2b Isotype
CDV-NP
Canine Distemper Virus monoclonal antibody specific for nucleoprotein (inclusions). IgG2B isotype (kappa light chain). Cross-reacts with measles virus. Does not react with Canine Adenovirus Type 2 (CAV-2), Canine Coronavirus (CCV), Canine Parainfluenza Virus (CPI), or Canine Parvovirus (CPV). The concentration is 1.0 mg/ml in phosphate-buffered saline, preserved with sodium azide. Suitable for immunofluorescence, western blot, immunohistochemistry, capture ELISA. Immunofluorescence staining is cytoplasmic only (no nuclear staining) and is intense; inclusion bodies are stained most intensely. Using ultracentrifuge-precipitated CDV in reducing gels, it Western blots giving major bands at 76, 68, 54 and 32 kD. Immunohistochemistry has been performed using paraffin-embedded tissues to detect CDV.
SHELF LIFE:
One year from date of sale
STORAGE:
Store at 2-7°C. Do not freeze!
SIZE:
0.1 mg
ORIGIN:
Mouse Ascites
INFECTIOUS AGENT:
canine distemper
RESTRICTIONS:
NA
KNOWN APPLICATIONS:
Immunofluorescence, Western blot, Immunohistochemistry, ELISA
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Ramos-Vara, J.A., and M.E. Beissenherz. Optimization of immunohistochemical methods using two different antigen retrieval methods on formalin-fixed paraffin-embedded tissues: Experience with 63 markers. J. Vet. Diagn. Invest. 12(4):307-311 (July 2000).
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Stanton, J.B., et al. Development of a semi-nested reverse transcription polymerase chain reaction assay for the retrospective diagnosis of canine distemper virus infection. J. Vet. Diagn. Invest. 14(1):47-52 (Jan. 2002).
