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Anaplasma Antibody Test Kit, cELISA | |
| Catalog No.: |
282-5 |
| Size: |
5 plates • maximum 455 samples |
| Availability: |
Readily available. |
| Description: |
This Anaplasma Antibody Test Kit is a competitive enzyme-linked immunosorbent assay (cELISA) for the detection of antibodies specific for Anaplasma in bovine serum samples. It is intended to provide results which will give guidance about the presence of Anaplasma infection in cattle. The principle of the test is as follows: Sample serum antibodies to Anaplasma inhibit the binding of a horseradish peroxidase (HRP)-labeled monoclonal antibody to the Anaplasma antigen coated on the plastic wells. Binding, or lack of binding, of the HRP-labeled monoclonal antibody conjugate is detected by the addition of enzyme substrate and quantified by subsequent color product development. Strong color development indicates little or no blockage of HRP-labeled monoclonal antibody binding and therefore the absence of antibodies to Anaplasma in the sample serum. Weak or no color development due to inhibition of the monoclonal antibody binding to the antigen on the solid phase indicates the presence of Anaplasma antibodies in the sample serum. |
| Format: |
cELISA, stripwell |
| Expiration: |
Eighteen months from date of QC release. |
| Storage: |
Store at 2-8°C. Do not freeze! |
| Disclaimer: |
For Veterinary Use Only - Use Only As Directed |
References
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Instructions for Use (pdf)
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Bovine Anaplasmosis in OIE International Animal Health Code
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N. I. Strik, et al Characterization of Anaplasma phagocytophilum Major Surface Protein 5 and the Extent of Its Cross-Reactivity with A. marginale. Clin Vaccine Immunol. 14(3): 262–268 (March 2007).
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Knowles, D., et al. Antibody against an Anaplasma marginale MSP5 epitope common to tick and erythrocyte stages identifies persistently infected cattle. J. Clin. Microbiol. 34(9):2225-2230 (Sept. 1996).
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Herrero, M.V., et al. Prospective study for the detection of Anaplasma marginale Theiler, 1911 (Rickettsiales: Anaplasmataceae) in Costa Rica. Ann. N.Y. Acad. Sci. 849:226-233 (June 1998).
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Bovine Anaplasmosis in OIE Manual for Diagnostic Tests and Vaccines
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Optimal Plate Washer Settings For the Bio-Tek Elx 50
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Product Information Sheet
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Torioni de Echaide, S., et al. Detection of cattle naturally infected with Anaplasma marginale in a region of endemicity by nested PCR and a competitive enzyme-linked immunosorbent assay using recombinant major surface protein 5. J. Clin. Microbiol. 36(3):777-782 (March 1998).
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Torioni de Echaide, S., et al. Detection of cattle naturally infected with Anaplasma marginale in a region of endemicity by nested PCR and a competitive enzyme-linked immunosorbent assay using recombinant major surface protein 5. Erratum. J. Clin. Microbiol. 39(3):1207a (March 2001).
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Coetzee JF, et al. Comparison of the complement fixation test and competitive ELISA for serodiagnosis of Anaplasma marginale infection in experimentally infected steers. Am J Vet Res. 68(8):872-8 (Aug 2007).
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U. M. Dreher, et al. Serologic Cross-Reactivity between Anaplasma marginale and Anaplasma phagocytophilum Clin Diagn Lab Immunol. 12(10): 1177–1183 (October 2005).
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Hoar BR, et al. Evaluation of sequential coinfection with Anaplasma phagocytophilum and Anaplasma marginale in cattle. J Am Vet Med Assoc. 1;233(5):742 (Sep 2008).
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Optimal Plate Washer Settings For the Bio-Tek Elx 50
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