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Neospora caninum Antibody Test Kit, cELISA | |
| Catalog No.: |
280-2 |
| Size: |
2 plates • maximum 184 samples |
| Availability: |
Readily available. |
| Description: |
This competitive enzyme-linked immunosorbent assay (cELISA) detects antibodies to Neospora caninum in sera of infected cattle. Sample serum Neospora caninum antibody inhibits binding of horseradish peroxidase (HRP)-labeled Neospora caninum-specific monoclonal antibody to Neospora caninum tachyzoite antigen coated on the plastic wells. Binding of the HRP-labeled monoclonal antibody conjugate is detected by the addition of enzyme substrate and quantified by subsequent color product development. Strong color development indicates little or no blockage of HRP-labeled monoclonal antibody binding and therefore the absence of Neospora caninum antibody in sample sera. Weak color development due to inhibition of the monoclonal antibody binding to the antigen on the solid phase indicates the presence of Neospora caninum antibodies in sample sera. |
| Format: |
cELISA, stripwell |
| Expiration: |
Fifteen months from date of QC release. |
| Storage: |
Store at 2-7°C. Do not freeze! |
| Disclaimer: |
For Veterinary Use Only - Use Only As Directed |
References
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Instructions for use (pdf)
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Product Information Sheet
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Baszler, T.V., et al. Validation of a commercially available monoclonal antibody-based competitive-inhibition enzyme-linked immunosorbent assay for detection of serum antibodies to Neospora caninum in cattle. J. Clin. Microbiol. 39(11):3851-3857 (Nov. 2001).
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Baszler, T.V., et al. Serological diagnosis of bovine neosporosis by Neospora caninum monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay. J. Clin. Microbiol. 34(6):1423-1428 (June 1996).
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Larson, R.L., et al. Economic considerations for diagnostic and control options for Neospora caninum-induced abortions in endemically infected herds of beef cattle. J. Am. Vet. Med. Assoc. 224(10):1597-1604 (May 2004).
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Jakubek, E-B., and A. Uggla. Persistence of Neospora caninum-specific immunoglobulin G antibodies in bovine blood and lung tissue stored at room temperature. J. Vet. Diagn. Invest. 17(5):458-460 (Sept. 2005).
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Waldner, C.L., et al. Agreement between three serological tests for Neospora caninum in beef cattle. J Vet Diagn Invest. 2004 Jul;16(4):313-5.
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Optimal Plate Washer Settings For the Bio-Tek Elx 50
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Millan J., et al. Seroprevalence of Toxoplasma gondii and Neospora caninum in feral cats (Felis silvestris catus) in Majorca, Balearic Islands, Spain. Vet Parasitol. 2009 Nov 12;165(3-4):323-6. Epub 2009 Jul 15.
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